nupG [2017-05-24 10:21:41]

purine nucleoside transporter

locus: BSU39020

pI: 7.76

mw: 43.56 kDa

protein length: 397 aa

gene length: 1191 bp

function: purine uptake

product: purine nucleoside transporter

essential: no

synonyms: yxjA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,005,752 → 4,006,945

Phenotypes of a mutant

• a ''[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]

The protein

Protein family

• SLC28A transporter family (according to Swiss-Prot)

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]

  regulatory mechanism

  • [regulon|G-box|G-box]: termination, [Pubmed|12923093], in [regulon|G-box|G-box]

  regulation

  • expression activated by glucose (4.3 fold) [Pubmed|12850135]
  • the [SW|G-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B725 (yxjA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1724 NBRP B. subtilis, Japan]
• CS219 (''[gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]

References

• 12850135,18763711,12923093,21926227,27197833